survey of solvents extraction of grape seed extracts and assay of antimicrobial properties

this study has been done to survey of solvent system effect on extraction of grape seed extract and its antimicrobial properties. for this purpose, grape seed extracts were extracted with six different solvent systems. minimum bactericidal concentration (mbc) of extract on six spoilage and food-born pathogens was determined. the results indicated that mbc of different extracts have no significant difference and its value for salmonella enteritidis, escherichia coli, enterobacter aerogenes was 1000 ppm. this value for s.aureus and listeria monocitogenes was 500 ppm. results showed that gram positive bacteria were more sensitive than gram negative ones.